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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Norte / UEP-Parnaíba. |
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Data corrente: |
01/11/1995 |
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Data da última atualização: |
01/11/1995 |
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Autoria: |
CHOI, P. S.; SOH, W. Y.; KIM, Y. S.; YOO, O. J.; LIU, J. R. |
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Título: |
Genetic transformation and plant regeneration of watermelon using Agrobacterium tumefaciens. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Plant Cell Reports, v.13, n.6, p.344-348, 1994. |
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Idioma: |
Inglês |
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Conteúdo: |
Adventitious shoots formed on the proximal cut edges of different cotyledonary explants of watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai; cvs. Sweet Gem and Gold Medal] cultured on Murashige and Skoog's (MS) medium with 1 mgl-1 6-benzyladenine (BA). Light (16-h photoperiod, about 7 Wm-2 cool-white fluorescent lamps) was essential for shoot formation. To obtain transformed plants, cotyledonary explants of "Sweet Gem" were cocultured with Agrobacterium tumefaciens LBA4404, a disarmed strain harboring a binary vector pBI121 carrying the CaMV 35S promoter-belta-glucuronidase (GUS) gene fusion used as a reporter gene and NOS promoter-neomycin phosphotransferase gene as a positive selection marker, for 48h on MS medium with 1 mgl-1 BA and 200 uM beta-hydroxyacetosyringone. After 48h of culture, explants were transferred to medium with 1 mgl-1 BA, 250 mgl-1 carbenicillin, and 100 mgl-1 kanamycin and cultured in the light. Adventitious shoots formed on the explants after 4 weeks of culture. When subjected to GUS histochemical assay, young leaves obtained from the shoots showed a positive response at a frequency of up to 16%. Preculturing cotyledonary explants on MS medium with 1 mgl-1 BA for 5d enhanced the competence of the cells to be transformed by Agrobacterium Southern blot analysis confirmed that the GUS gene was incorporated into the genomic DNA of the GUS-positive regenerants. The transformed plants were grown to maturity. |
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Palavras-Chave: |
Melhoramento genetico; Watermelon. |
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Thesagro: |
Agrobacterium Tumefaciens; Melancia. |
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Thesaurus Nal: |
genetic transformation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02095naa a2200229 a 4500
001 1075311
005 1995-11-01
008 1994 bl --- 0-- u #d
100 1 $aCHOI, P. S.
245 $aGenetic transformation and plant regeneration of watermelon using Agrobacterium tumefaciens.
260 $c1994
520 $aAdventitious shoots formed on the proximal cut edges of different cotyledonary explants of watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai; cvs. Sweet Gem and Gold Medal] cultured on Murashige and Skoog's (MS) medium with 1 mgl-1 6-benzyladenine (BA). Light (16-h photoperiod, about 7 Wm-2 cool-white fluorescent lamps) was essential for shoot formation. To obtain transformed plants, cotyledonary explants of "Sweet Gem" were cocultured with Agrobacterium tumefaciens LBA4404, a disarmed strain harboring a binary vector pBI121 carrying the CaMV 35S promoter-belta-glucuronidase (GUS) gene fusion used as a reporter gene and NOS promoter-neomycin phosphotransferase gene as a positive selection marker, for 48h on MS medium with 1 mgl-1 BA and 200 uM beta-hydroxyacetosyringone. After 48h of culture, explants were transferred to medium with 1 mgl-1 BA, 250 mgl-1 carbenicillin, and 100 mgl-1 kanamycin and cultured in the light. Adventitious shoots formed on the explants after 4 weeks of culture. When subjected to GUS histochemical assay, young leaves obtained from the shoots showed a positive response at a frequency of up to 16%. Preculturing cotyledonary explants on MS medium with 1 mgl-1 BA for 5d enhanced the competence of the cells to be transformed by Agrobacterium Southern blot analysis confirmed that the GUS gene was incorporated into the genomic DNA of the GUS-positive regenerants. The transformed plants were grown to maturity.
650 $agenetic transformation
650 $aAgrobacterium Tumefaciens
650 $aMelancia
653 $aMelhoramento genetico
653 $aWatermelon
700 1 $aSOH, W. Y.
700 1 $aKIM, Y. S.
700 1 $aYOO, O. J.
700 1 $aLIU, J. R.
773 $tPlant Cell Reports$gv.13, n.6, p.344-348, 1994.
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Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP) |
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| 1. |  | BENAVIDES, M. V.; MILANO, L. R.; MUÑOZ, F. P.; FRANCK, B. M.; ILHA, G. C.; ALMEIDA, L. S.; CORRÊA, A. P. R.; SACCO, A. M. S. Identificação do estado de portador sadio de Babesia bigemina em bovinos através da técnica de PCR In: CONGRESSO BRASILEIRO DE GENÉTICA, 50., 2004, Florianópolis. 50 anos desvendando a genética: resumos. Florianópolis: SGB, 2004. p. 837.| Tipo: Resumo em Anais de Congresso |
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